Yeast display
protein engineering technique
Yeast display (or yeast surface display) is a protein engineering technique that uses the expression of recombinant proteins incorporated into the cell wall of yeast. This method can be used for several applications such as isolating and engineering antibodies and determining host-microbe interactions.
Development
The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup and Eric T. Boder. The technology was sold to Abbott Laboratories in 2001.
How it works
A protein of interest is displayed as a fusion to the Aga2p protein on the surface of yeast. The Aga2p protein is used by yeast to mediate cell–cell contacts during yeast cell mating. As such, display of a protein via Aga2p likely projects the fusion protein from the cell surface, minimizing potential interactions with other molecules on the yeast cell wall. The use of magnetic separation and flow cytometry in conjunction with a yeast display library can be highly effective method to isolate high affinity protein ligands against nearly any receptor through directed evolution.
Advantages and disadvantages
Advantages of yeast display over other in vitro evolution methods include eukaryotic expression and post translational processing, quality control mechanisms of the eukaryotic secretory pathway, minimal avidity effects, and quantitative library screening through fluorescent-activated cell sorting (FACS). Yeast are eukaryotic organisms that allow for complex post-translational modifications to proteins that no other display libraries are able to provide.
The public source identifies “Yeast display” as protein engineering technique. This brief keeps that definition visible, then builds a research path around Yeast, display and protein.
Why this record matters
A short description can identify a subject without explaining its stakes. For “Yeast display”, the useful work is to connect “protein engineering technique” to the records capable of establishing context and consequence.
The citation trail is more important than the brevity of the summary: it shows where individual claims can be examined in context. The source revision retrieved here is dated Oct 11, 2025. The linked authority identifier is Q8051003. None of the 0 selected statements returned an explicit reference. The first chronological checks are 2001.
A concise general-reference account can conceal disagreements about scope, terminology or the weight assigned to individual sources. The lead is largely declarative, so disagreement and counter-evidence require a deliberate search beyond the opening account. Authority statements aid reconciliation but still require their own references, qualifiers and ranks to be checked.
How to read it
Use the entry as an orientation point, then follow its citations and revision history. Names, dates and institutional relationships should be checked against the original record.
- Subject orientation
- Search vocabulary
- Locating named sources
The closest primary source, responsible institution and strongest cited specialist reference.
Three-step research path
- Establish the record: confirm the title “Yeast display”, its source revision and the description used here.
- Expand the search: follow Yeast display primary sources, Yeast display archive and Yeast research across catalogues and specialist indexes.
- Test the account: compare the strongest cited source with the responsible institution’s current record and note any disagreement.
Questions for further research
- Which source most directly establishes the central claim about “Yeast display”?
- Which cited source is closest to the event, object or claim?
- Which institution is responsible for the underlying evidence?
Search terms from this dossier
This entry incorporates text from “Yeast display” on English Wikipedia. Contributors are listed in the page history. Text is available under the Creative Commons Attribution-ShareAlike 4.0 License. Selected authority identifiers and statements are retrieved from Wikidata under CC0; their references and qualifiers remain part of the verification path.