Kompetitive allele specific PCR
term
Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific oligo extension and fluorescence resonance energy transfer for signal generation.
A single-nucleotide polymorphism (SNP) occurs when a single nucleotide in a DNA sequence differs between members of the same species or a paired chromosome. SNPs work as molecular markers that help locate genes associated with disease and are used for genotype sequencing.
Genotyping by next generation sequencing using SNPs is expensive, time-consuming, and has some missing data. There are many other SNP techniques that can be used depending on the purpose of the research considering throughput, data turnaround time, ease of use, performance (sensitivity, reliability, reproducibility, accuracy) flexibility, requirements, and cost. For the highest throughput for large scale studies, it is best to choose multiplexed chip-based technology. Multiplex technologies generate anywhere from 100 to over a million SNPs per run but are not economical to use for small to moderate numbers of SNPs. For a smaller number of SNPs, a uniplex assay like KASP can be used.
Methodology
There are three components that are critical to the KASP assay: 1) a purified DNA sample, 2) two allele-specific forward primers, and 3) a common reverse primer.
This brief starts where responsible research should: with the source description of “Kompetitive allele specific PCR” as term. Everything that follows is an evidence route, not borrowed authority.
Why this record matters
The subject matters to the general reference register because the source frames it as term. Its deeper value depends on whether names, dates, institutions and citations support that framing.
The citation trail is more important than the brevity of the summary: it shows where individual claims can be examined in context. The source revision retrieved here is dated Jul 11, 2026. The linked authority identifier is Q17148584. None of the 0 selected statements returned an explicit reference.
The absence of detail may reflect summary conventions rather than a lack of surviving documentation. The lead is largely declarative, so disagreement and counter-evidence require a deliberate search beyond the opening account. Authority statements aid reconciliation but still require their own references, qualifiers and ranks to be checked.
How to read it
Use the entry as an orientation point, then follow its citations and revision history. Names, dates and institutional relationships should be checked against the original record.
- Subject orientation
- Search vocabulary
- Locating named sources
The closest primary source, responsible institution and strongest cited specialist reference.
Three-step research path
- Establish the record: confirm the title “Kompetitive allele specific PCR”, its source revision and the description used here.
- Expand the search: follow Kompetitive allele specific PCR primary sources, Kompetitive allele specific PCR archive and Kompetitive research across catalogues and specialist indexes.
- Test the account: compare the strongest cited source with the responsible institution’s current record and note any disagreement.
Questions for further research
- Which source most directly establishes the central claim about “Kompetitive allele specific PCR”?
- Which cited source is closest to the event, object or claim?
- What terminology or title could unlock a more precise catalogue search?
Search terms from this dossier
This entry incorporates text from “Kompetitive allele specific PCR” on English Wikipedia. Contributors are listed in the page history. Text is available under the Creative Commons Attribution-ShareAlike 4.0 License. Selected authority identifiers and statements are retrieved from Wikidata under CC0; their references and qualifiers remain part of the verification path.