Expanded bed adsorption
Open-knowledge reference entry
Expanded bed adsorption (EBA) is a preparative chromatographic technique which makes processing of viscous and particulate liquids possible.
Principle
The protein binding principles in EBA are the same as in classical column chromatography and the common ion-exchange, hydrophobic interaction and affinity chromatography ligands can be used. After the adsorption step is complete, the fluidized bed is washed to flush out any remaining particulates. Elution of the adsorbed proteins was commonly performed with the eluent flow in the reverse direction; that is, as a conventional packed bed, in order to recover the adsorbed solutes in a smaller volume of eluent. However, a new generation of EBA columns has been developed, which maintain the bed in the expanded state during this phase, producing high-purity, high yields of e.g. MAbs [monoclonal antibodies] in even smaller volumes of eluent. Process duration at manufacturing scale has also been cut considerably (under 7 hours in some cases).
EBA may be considered to combine both the "Removal of Insolubles" and the "Isolation" steps of the 4-step downstream processing heuristic. The major limitations associated with EBA technology is biomass interactions and aggregations onto adsorbent during processing.
Where classical column chromatography uses a solid phase made by a packed bed, EBA uses particles in a fluidized state, ideally expanded by a factor of 2.
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