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3,5-Difluoro-4-hydroxybenzylidene imidazolinone

chemical compound

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Science and natureInterpretive dossier study · Crown Archives visual atlas
Record originEnglish Wikipedia
Text licenseCC BY-SA 4.0
Source revisionDec 13, 2025
Entity authorityQ27453616 ↗
Source-derived summary

3,5-Difluoro-4-hydroxybenzylidene imidazolinone or DFHBI is an imidazolinone fluorophore used in various biochemical studies. The benzene ring of DFHBI can freely rotate around the single bond but when it is fixed in planar conformation, DFHBI fluoresces. It is a synthetic analog of the GFP fluorophore.

Editorial summary

“3,5-Difluoro-4-hydroxybenzylidene imidazolinone” enters the record as chemical compound. Crown Archives preserves that source wording while asking what 5-Difluoro-4-hydroxybenzylidene, imidazolinone and chemical can confirm, complicate or overturn.

Editorial reviewA practical orientation to terminology and classification, particularly when read beside dated observations, specimens or technical literature. The current 44-word lead offers orientation but no explicit four-digit date, so chronology should not be assumed. The selected authority fields contribute no independent date. Its strongest next move is a source search built around 5-Difluoro-4-hydroxybenzylidene, imidazolinone and chemical.
Editorial analysis

Why this record matters

“3,5-Difluoro-4-hydroxybenzylidene imidazolinone” is worth following because a concise public description often conceals a longer documentary argument. Here, 5-Difluoro-4-hydroxybenzylidene, imidazolinone and chemical provides the most credible route into that argument.

Evidence profile

Datasets, specimens, observations and peer-reviewed methods provide the appropriate test for the technical claims summarized here. The source revision retrieved here is dated Dec 13, 2025. The linked authority identifier is Q27453616. None of the 0 selected statements returned an explicit reference.

Critical limits

A general summary may omit uncertainty, sample limits or methodological disagreement that is explicit in the technical record. The lead is largely declarative, so disagreement and counter-evidence require a deliberate search beyond the opening account. Authority statements aid reconciliation but still require their own references, qualifiers and ranks to be checked.

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Source & attribution

This entry incorporates text from “3,5-Difluoro-4-hydroxybenzylidene imidazolinone” on English Wikipedia. Contributors are listed in the page history. Text is available under the Creative Commons Attribution-ShareAlike 4.0 License. Selected authority identifiers and statements are retrieved from Wikidata under CC0; their references and qualifiers remain part of the verification path.