Xylene cyanol
chemical compound
Xylene cyanol can be used as an electrophoretic color marker, or tracking dye, to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose.
Once mixed with the sample, the concentration of xylene cyanol is typically about 0.005% to 0.03%.
Migration speed
In 1% agarose gels, xylene cyanol migrates at about the same rate as a 4 to 5 kilobase pair DNA fragment, although this depends on the buffer used. Xylene cyanol on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment. On 20% denaturating (7 M urea) polyacrylamide gel electrophoresis (PAGE), xylene cyanol migrates at about the rate of 25 bases oligonucleotide.
“Xylene cyanol” enters the record as chemical compound. Crown Archives preserves that source wording while asking what Xylene, cyanol and chemical can confirm, complicate or overturn.
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Datasets, specimens, observations and peer-reviewed methods provide the appropriate test for the technical claims summarized here. The source revision retrieved here is dated Jul 27, 2025. The linked authority identifier is Q2734627. None of the 0 selected statements returned an explicit reference.
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