BOD bottle
scientific device
BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.
Structure
The bottle is normally designed to have a special shoulder radius to push out all air from the inside of the bottle when a sample solution is being filled. According to Method 5210 in Standard Methods for the Examination of Water and Wastewater, the BOD bottle should include a ground-glass stopper and a flared mouth which form a water seal preventing the air from the outside of the bottle coming in. Method 5210 also recommends to use a paper, a foil or a plastic cup to cap over the mouth of the bottle reducing the evaporation during the incubation. Generally, the side of the BOD bottle is permanently screened with white writing area, and is printed with a specific number; both for the aid of the sample identification.
Stopper
There are two kinds of stopper: the Glass Pennyhead and the Glass Robotic stopper.
Sizes
There are many BOD bottle sizes. The dose of the mixture of the solution (nutrient, mineral and buffer solution) is related to the size of the bottle. For the Standard Methods 5210, the BOD bottle “having 60 mL or greater capacity (300-mL)” is mentioned as one of the apparatus for the BOD test.
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This entry incorporates text from “BOD bottle” on English Wikipedia. Contributors are listed in the page history. Text is available under the Creative Commons Attribution-ShareAlike 4.0 License. Selected authority identifiers and statements are retrieved from Wikidata under CC0; their references and qualifiers remain part of the verification path.